摘要
本研究以长白猪(Landrace)肌肉cDNA为模板,克隆得到长白猪Akt3基因.序列分析结果显示:长白猪Akt3基因cDNA全长1493bp,编码一个含479个氨基酸的前体蛋白,与人、牛、小鼠和大鼠的氨基酸序列一致性分别为100%、100%、99.58%、99.79%,具有高度保守性.氨基酸结构分析显示,长白猪Akt3基因都具有典型的Pleckstrin homologous domain(PH)结构域和丝氨酸/苏氨酸磷酸化激活位点.组织表达图谱分析结果显示:Akt3基因在所有检测的家猪组织中均有表达.此外,本研究还将Akt3基因编码区序列克隆转入真核表达载体pcDNA3.1(+)中,为下一步功能验证做准备.
In this study, using cDNA of Landrace pig muscle as the template, the full-length cDNA for Akt3 gene was cloned. The Akt3 cDNA was 1493bp in length and encodes a protein of 479 amino acids, which shares high amino acid sequence identity with that of human （100%）, cattle （100%）, mouse （99. 58%） and rat （99.79%）. Structural analyses indicate that pig Akt3 contains a PH domain （Pleckstrin homologous domain） and a serine/threonine phosphorylation site. RT-PCR assay showed that the Akt3 was expressed in all tissues examined. In addition, the eukaryotic expression vector for Akt3 has been constructed for further functional analyses.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第4期815-820,共6页
Journal of Sichuan University(Natural Science Edition)
基金
国家重大专项转基因生物新品种培育(2009zx08009-145B)
