摘要
目的研制大黄酚前体脂质体并对其质量考察。方法采用载体沉积法制备大黄酚前体脂质体,以包封率为评价指标,通过单因素考察和正交试验法优化该处方,并观察其稳定性、形态及粒径。结果大黄酚前体脂质体的最佳处方:温度为40℃,载脂比为40∶1,胆固醇卵磷脂比为1∶5,药脂比1∶12;按该处方制备的大黄酚前体脂质体稳定性好,包封率达(84.69±2.29)%,平均粒径为(593.4±14.2)nm,Zeta电位为(-54.35±0.88)mV。结论采用载体沉积法,以山梨醇为载体,按最优处方和最佳制备条件可制得包封率较高、稳定性好、粒径均匀的大黄酚前体脂质体。
Objective To study and evaluate the quality of chrysophanol proliposomes. Methods Chrysophanol proliposomes were prepared by carrier aggradation method. A single-factor investigation and orthogonal design were used to optimize the formulation by entrapment efficiency （EE）. And the stability, shape, and size were observed. Results The optimum prescription of chrysophanol proliposomes as follows： the temperature was 40 ℃, the ratio of carrier-phospholipid was 40 ： l, the ratio of cholesterol-phospholipid was 1 ： 5, and the ratio ofphospholipid-drug was 1 ： 12. Chrysophanol proliposomes prepared by the result of the orthogonal design showed the uniform particle and good stability with the EE up to （84.69 ± 2.29）%, the particle size was （593.4 ± 14.2） nm, and the Zeta potential was （-54.35 ± 0.88） mV. Conclusion The proliposomes prepared by the carrier aggradation method are used by sorbitol to get high EE, stability and uniform particle size to optimize the prescription and preparation conditions.
出处
《中草药》
CAS
CSCD
北大核心
2014年第14期2014-2017,共4页
Chinese Traditional and Herbal Drugs
基金
河北省科学技术研究与发展计划项目(12276104D-94)
河北北方学院应用化学创新团队资助项目(CXTD1306)
关键词
大黄酚
前体脂质体
载体沉积法
制备
质量评价
包封率
chrysophanol
proliposome
carrier aggradation method
preparation
quality evaluation
entrapment efficiency
