摘要
本文从显色培养基的特性、灵敏度、特异性、抗干扰性和耗时等各方面比较GB 4789.4-2010、API 20E、VITEK和mini VIDAS四种方法在食品中检测沙门氏菌的效果。显色培养基的分离选择性直接影响四种方法的检测结果。研究结果表明,BS和RS培养基的选择性较好,沙门氏菌在XLD和BS培养基上生长较好。mini VIDAS的检出限为105CFU/mL,SX2作为mini VIDAS的增菌肉汤检测效果优于TTB和SC。四种方法都能排除6种阴性对照菌株的干扰,但当沙门氏菌与宋内氏志贺氏菌混合时,XLD和HE培养基能筛选出可疑菌,其他培养基和mini VIDAS会出现假阴性结果。
The examination effect of Salmonella in food with GB 4789.4-2010, API 20E, VITEK and mini VIDAS was compared with the following aspects ：the properties of Salmonella chromogenic medium, sensibility, specificity, anti -interference and time consuming.The separation effect of chromogenic medium impacted the examination effect directly.The results indicated BS and RS had better selectivity,while Salmonella grew better on XLD and BS.The detection limits of mini VlDAS was 10^5CFU/mL. SX2 was better than TTB and SC as enrichment broth of mini VIDAS.Four methods all could exclude the interference of six negative control strains.When Salmonella mixed with Shigella soonei,XLD and HE could separate suspicious bacteria,other methods reported false negative.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第15期185-187,192,共4页
Science and Technology of Food Industry
