摘要
目的 探讨蛋白激酶Cα相互作用蛋白1（PICK1）在结直肠腺癌中的表达及其意义.方法 采用免疫组织化学及Western blot方法检测PICK1在结直肠腺癌、腺瘤、增生性息肉及癌旁组织中的表达,并分析其与临床病理参数的关系.结果 免疫组织化学结果显示,102例结直肠腺癌组织中PICK1的阳性表达率为72.5％（74/102）,其中高表达率为30.4％（31/102）,腺瘤组织中的PICK1高表达率为22.2％（8/36）,较癌旁组织中的高表达率0（0/40）增高（P＜0.05）;36例增生性息肉组织PICK1的高表达率为5.6％（2/36）,与癌旁组织比较差异无统计学意义（P＞0.05）.Western blot结果显示,PICK1在结直肠腺癌组织中的表达（1.10±0.04）明显高于癌旁组织（0.75±0.07）（P＜0.05）.在结直肠腺癌组织中,PICK1的高表达与肿瘤的发生部位、分化程度、浸润深度、TNM分期及淋巴结转移有关（均P＜0.05）,与年龄及性别无关（均P＞0.05）.结论 PICK1与结直肠腺癌的发生、发展和侵袭转移有关,并有助于患者预后的判断.
Objective To investigate the expression of protein interacting with Cα kinase 1 （PICK1） and its significance in colorectal adenocarcinoma.Methods The expression of PICK1 were detected by immunohistochemistry and Western blot methods in tissues of colorectal adenocarcinoma,colorectal adenoma,colorectal polyp and adjacent tissues.The correlation between PICK1 and clinical pathological data was explored.Results Immunohistochemical assay showed that the positive ratio of PICK1 protein was 72.5 % （74/102）,and overexpressed in 31 cases （30.4 %,31/102） with colorectal adenocarcinoma.The ratio of high expression level of PICK1 in colorectal adenoma tissues （22.2 %,8/36） was significantly higher than that in the adjacent tissues （0,0/40） （P 〈 0.05）.The ratio of high expression level of PICK1 in colorectal polyp tissues （5.6 %,2/36） was no statistically difference compared with that of the adjacent tissues （P 〉 0.05）.Western blot analysis revealed that the expression of PICK1 in colorectal adenocarcinoma （1.10±0.04） was significantly higher than that in the adjacent tissues （0.75±0.07） （P 〈 0.05）.The result showed significant correlation with the tumor location,the degree of differentiation,depth of invasion,TNM stages and lymph metastasis （all P 〈 0.05）.However,there is no correlation was revealed between PICK1 expression and the patients age,gender （both P 〉 0.05）.Conclusion The expression of PICK1 may be associated with the tumorgenesis,progression,invasion and metastasis of colorectal adenocarcinoma,which contributes to the prognosis of patients.
出处
《肿瘤研究与临床》
CAS
2014年第6期373-376,380,共5页
Cancer Research and Clinic
基金
山西省自然科学基金（2013011043-2）
