摘要
目的 研究比较4种不同来源木聚糖酶的酶学性质,为各酶在不同领域应用提供理论依据.方法 通过摇瓶发酵获得源自4种菌株的木聚糖酶粗酶液,用二硝基水杨酸（DNS）法测定各木聚糖酶的酶活性,并研究酶学性质.结果 嗜热棉毛菌、绿色木霉、泡盛曲霉和橄榄绿链霉菌来源的木聚糖酶的最适温度分别为70,60,50和60℃;最适pH分别为6,4,4和6.嗜热棉毛菌来源的木聚糖酶耐热性最强,高温60℃下,酶活性高于85％,70℃仍保持60％以上的酶活性,且在pH4～11的范围内能保持较高稳定性.另外3种来源的木聚糖酶则有较强的耐酸性.结论 嗜热棉毛菌来源的木聚糖酶有饲料添加剂方面的应用优势.另外3种来源的木聚糖酶可根据不同行业对木聚糖酶的要求,应用于食品、医药、能源、环境等领域.
Objective To study and compare the enzymatic properties of xylanase from four different strains to provide a theoretical basis for xylanase applications in various social fields.Methods Xylanase crude enzyme solutions from the four kinds of strains were obtained by fermentation.Xylanase activity was measured with DNS method and xylanase enzymatic properties from different sources were studied.Results The optimum temperature of xylanase from Thermomyces lanuginosus,Trichoderma viride,Aspergillus awamori and Streptomyces olivoviridis was 70 ℃、60 ℃、50 ℃ and 60 ℃,respectively.And the optimum pH of xylanase was 6,4,4 and 6,respectively.Xylanase from Thermomyces lanuginosus exhibited the strongest heat resistance,more than 85％ xylanase activity was detected under 60 ℃,and more than 60 ％ activity was obtained when 70 ℃ was used.Furthermore,this xylanase can maintain high stability in the broad range of pH 4-11.The other three xylanase all showed strong acid-resistance.Conclusion Xylanase from Thermomyces lanuginosus exhibits its application advantages as a feed additive.The other three xylanase can be applied in food,medicine,energy,environment and other fields according to the relevant requirements of xylanase.
出处
《食品与药品》
CAS
2013年第6期397-399,共3页
Food and Drug
关键词
木聚糖酶
嗜热棉毛菌
绿色木霉
泡盛曲霉
橄榄绿链霉菌
xylanase
Thermomyces lanuginosus
Trichoderma viride
Aspergillus awamori
Streptomyces olivoviridis
