摘要
目的:观察和分析冠心病患者抗心磷脂抗体(ACA)与血清对氧磷酶(PON)活性、氧化低密度脂蛋白(oxLDL)、丙二醛(MDA)和超氧化物歧化酶(SOD)的相关性。方法:酶联免疫法检测118例冠心病患者、68例正常对照者血清ACA、ox-LDL、硫代巴比妥酸法测定MDA、黄嘌呤氧化酶法测定SOD、乙酸苯酯法测定PON。结果:冠心病患者ACA阳性率为24%,显著高于对照组(阳性率为9%,P<0.05)。ACA阳性冠心病患者PON活性为(122±40)kU/L、ox-LDL为(0.81±0.26)mg/L、MDA为(7.6±1.1)μmol/L、SOD为(72±20)μU/L,与ACA阴性冠心病相比PON活性降低,ox-LDL、MDA升高,SOD无明显变化。相关分析显示PON活性与ox-LDL、MDA呈负相关(分别r=-0.825,P<0.01;r=-0.735,P<0.01),与SOD无相关性。结论:ACA阳性冠心病患者反映脂质过氧化程度的ox-LDL、MDA增高,而PON活性降低。
AIM： To observe and analyze the relationship between anticardiolipin antibody （ACA） and paraoxonase （PON）, oxidized low-density lipoprotein （ox-LDL）, malondialdehycle （MDA）, superoxide dismutase （SOD） in patients with coronary heart disease （CHD）. METHODS： ACA and ox-LDL were measured by enzyme-linked immunosorbent assay, MDA by thiobarbiuric acid, SOD by xanthine superoxidase, PON by the method of phenyl acetate in 118 patients with CHD and 68 healthy persons. RESULT： The ACA positive rate of CHD was 24%, which was significantly higher than that of healthy persons （the ACA positive rate was 9%, P 〈0. 05） PON, ox-LDL, MDA, SOD levels were （122 ±40） kU/L, （0. 81± 0. 26） mg/L, （7.6 ± 1.1） μmol/L and （72±20） μU/ml, respectively, in ACA positive CHD. PON was significantly decreased （P 〈 0. 05）, ox-LDL and MDA levels were significantly increased （ P 〈 0. 05）, but SOD was not different compared with ACA negative CHD. There was a negative correlation between PON and ox-LDL, MDA by correlation analysis, respectively （r = -0. 825, P 〈0. 01 ; r = -0. 735, P 〈0. 01 ）. CONCLUSION： Lipid peroxidation levels （ox-LDL, MDA） were increased and the levels of PON were decreased in ACA-positive CHD.
出处
《心脏杂志》
CAS
2014年第4期453-455,共3页
Chinese Heart Journal
