摘要
目的:研究Irisin对2型糖尿病(type 2 diabetes mellitus,T2DM)小鼠血糖的影响及机制。方法:采用喂养高脂饮食结合腹腔注射小剂量的链脲佐菌素(STZ)建立T2DM小鼠模型。Western blot检测Irisin经腹腔注射后的血浆水平。血糖仪检测T2DM小鼠经腹腔注射Irisin后30、60、90、120、150、180和210 min的血糖水平。Western blot检测注射Irisin后肝脏组织中葡萄糖-6-磷酸酶(G6pase)和磷酸烯醇式丙酮酸羧化酶(PEPCK)的表达。结果:1与正常饮食(ND)组相比,高脂饮食(HD)组小鼠在第6周时血糖糖水平明显增加(P<0.05),且葡萄糖耐量降低,说明T2DM小鼠造模成功。2与对照(Control)组相比,Control+Irisin组血浆中的Irisin蛋白水平明显升高(P<0.05)。3与T2DM+Vehicle(生理盐水)组相比,T2DM+Irisin组血糖于30 min时开始降低,60 min时出现显著差异(P<0.05),到120 min时下降到最低点,以后逐渐升高。4与Control组相比,T2DM组G6pase和PEPCK的水平升高(P<0.05),经外源给予Irisin后,G6pase和PEPCK的水平下降(P<0.01)。结论:Irisin对T2DM小鼠具有降低血糖的作用,这可能与降低T2DM小鼠肝脏糖异生有关。
AIM： To investigate the effect of irisin on the metabolism of glucose in type 2 diabetic mice （T2DM） and its mechanism. METHODS： T2DM was induced by a high-fat diet （HD） plus low-dose streptozotocin （STZ） intraperitoneal injection. Blood irisin was determined after irisin intraperitoneal injection by Western blot evaluation. Blood glucose was determined at 30, 60, 90, 120, 150, 180 and 210 min in T2DM ＋ irisin group, respectively, by glucometer detection. The expressions of G6Pase and PEPCK were evaluated by Western blot. RESULTS： 1 ） Blood glucose increased 6 weeks after high-fat diet and glucose tolerance （IPGTF） decreased significantly in the diabetic mice. 2） Compared with control group, injections of irisin group plasma levels increased significantly （ P 〈 0. 01 ）. 3 ） Compared with T2DM group, in the group of intra-articular injections of normal saline, the level of blood glucose decreased 30 min after irisin ejection and reached its lowest level 120 min after irisin ejection （P 〈 0. 05）. The level of blood glucose was close to the basal level 210 min after irisin injection. 4） G6pase and PEPCK expressions increased in diabetic mice. Irisin decreased G6pase and PEPCK expressions in diabetic mice （P 〈0.01 ）. CONCLUSION： Irisin reduces instantaneous blood glucose level in type 2 diabetes mice possibly through decreasing hepatic gluconeogenesis.
出处
《心脏杂志》
CAS
2014年第4期408-410,415,共4页
Chinese Heart Journal
