摘要
以感染内生真菌的新疆和田一牧场小花棘豆为试验材料,通过建立内生真菌感染(E+)和未感染(E-)的小花棘豆植株,并对其进行氯化钠盐胁迫试验。在室内盆栽的条件下,比较带内生真菌E+与不带内生真菌E-的小花棘豆在相同环境下不同NaCl浓度(0、100、200、300 mmol/L)条件下脯氨酸、丙二醛、过氧化氢酶活性、过氧化物酶活性、可溶性糖、可溶性蛋白指标变化情况。试验结果表明:感染内生真菌植株的过氧化物酶、可溶性糖含量和可溶性蛋白含量都随盐胁迫浓度升高而升高,但感染内生真菌植株的过氧化物酶活性和可溶性蛋白含量均低于未感染植株;内生真菌感染的小花棘豆叶内脯氨酸含量在100、200 mmol/L,高于未感染植株;感染植株的过氧化氢酶活性呈先降低再升高的趋势,均低于非感染处理;但丙二醛的含量一直是感染植株低于未感染植株,且随盐分胁迫浓度增加,感染植株丙二醛含量呈现降低趋势。总体来看,内生真菌感染提高了宿主的抗盐性。
In this paper,Oxytropis Adabra infected by endophytie fungi was chosen as the salt-stress experimental material. Activities of POD, CAT and proline content, malondialdehyde, soluble sugar content, soluble protein content compared between E＋ and E- plants to study the contribution of the endophyte to improve the salt resistance of its host plant. The first factor was sustained salt stress with four levels of NaCl stress imposed ,which included the control （0 mmol/L NaCl concentrations ）, 100 mmol/L NaCl concentrations, 200 mmol/L NaCl concentrations and 300 mmol/L NaCl concentrations. The other factor was endophyte status, including E＋ and E-. Each treatment was replicated five times. The results showed that salt stress raised activities of POD ,soluble sugar and soluble protein content but the activities of POD and soluble protein content of E＋ leaves were lower while soluble sugar was higher when compared with E- leaves. Prolinc contents of E＋ leaves were higher than E- under 100 mmol/L NaCl and 200 mmol/L NaCl stress. The activities of CAT of E＋ leaves went up then went clown but all the treatments were lower than E-. Malondialdehyde content of E＋ leaves were lower than E-, and decreased under salt stress. As a whole, although endophyte didn＇t evidently enhanced the activity of POD and CAT,it did improve the responses of the host plant to salt stress.
出处
《现代农业科技》
2014年第13期287-289,共3页
Modern Agricultural Science and Technology
基金
塔里木大学大学生创新项目(2013107570032)
关键词
小花棘豆
宿主
内生真菌
盐胁迫
抗盐性
Oxytropis glabra
host
endophyte
salt stress
salt resistance
