摘要
将罗氏沼虾(Macrobrachium rosenbergii)在含0.1、0.2、0.4 mg/L三丁基锡(TBT)的水体中浸浴48 h,检测TBT对罗氏沼虾血清中部分免疫酶活力的影响。结果表明:12 h内高剂量(0.4 mg/L)可诱导超氧化物歧化酶(SOD),酶活力差异有统计学意义(P<0.01),至48 h时,诱导作用逐步解除;12 h内各剂量组血清中酸性磷酸酶(ACP)活力均表现抑制效应,酶活力差异有统计学意义(P<0.05),而高剂量组在48 h时TBT对ACP表现为诱导效应,酶活力高于对照组,差异有统计学意义(P<0.05);低剂量组(0.1 mg/L)TBT对罗氏沼虾血清中AKP活力的影响无统计学意义(P>0.05),而中剂量组(0.2 mg/L)和高剂量组(0.4 mg/L)AKP活力表现为先抑制后逐渐转为诱导的效应,。
The Macrobrachium rosenbergii was exposed to tributyltin （TBT） at different doses （0.1, 0.2, 0.4 mg/L） within 48 h, and effects of TBT on the activities of immunologic enzyme in blood serum was detected. Results showed that the activities of Superoxide dismutase （SOD） in serum were significantly increased （P 〈 0.05） at the high dose group within 12 h, and were decreased at 48 h. The activities of acid phosphates （ACP） in serum were significantly inhibited （P 〈 0.05） at each dose groups within 12 h, while the activities of ACP in serum were significantly increased （P 〈 0.05） at the high dose group at 48 h. The activities of alkaline phosphates （AKP） in serum were significantly decreased （P 〈 0.05） in the early stage at medium and high dose groups, but the effect on the activity of AKP in serum was no-significantly at the low dose group. The activities of AKP in serum were inhibited at first and then increased in medium and high dose groups.
出处
《广东海洋大学学报》
CAS
2014年第3期17-21,共5页
Journal of Guangdong Ocean University
基金
广东省自然科学基金(S2011010000254)
广东省科技计划(2008B040006)
关键词
罗氏沼虾
免疫酶
超氧化物歧化酶
酸性磷酸酶
碱性磷酸酶
三丁基锡
Macrobrachium rosenbergill
immunologic enzyme
superoxide dismutase （SOD）
acid phosphates （ACP）
alkaline phosphates （AKP）
tributyltin （TBT）
