摘要
目的观察体外冲击波疗法对激素性股骨头缺血坏死中血管内皮生长因子(VEGF)和骨形态发生蛋白(BMP)的影响。方法新西兰大白兔40只,Yamamoto法制造模成功后,左侧股骨头做冲击波治疗设为实验组(A组),右侧股骨头不做冲击波治疗设为对照组(B组)。A组分别于治疗后1、2、4、8、12周处死动物,每次随机处死8只,取双侧股骨头行大体标本HE染色及细胞计数,免疫组化观察VEGF和BMP的表达。结果 HE染色观察在体外冲击波冲击后4、8、12周,毛细血管计数平均值分别为9.95±2.14,14.70±3.08,13.15±3.07,而B组分别为4.85±1.67,4.30±1.63,4.40±1.79;成骨细胞计数A组平均值为33.35±3.39,47.35±5.20,53.40±5.09,而B组分别为24.1±2.45,21.70±4.08,19.60±4.28,HE染色两组比较差异均有统计学意义(P<0.05)。免疫组化染色在体外冲击波冲击后的2、4、8、12周VEGF表达A组平均值为0.104±0.018,0.177±0.020,0.167±0.017,0.144±0.031,而B组分别为0.074±0.017,0.078±0.021,0.078±0.023,0.070±0.019;且BMP表达A组平均值为0.210±0.028,0.304±0.039,0.292±0.052,0.268±0.053,而B组分别为0.136±0.025,0.140±0.033,0.144±0.029,0.118±0.028,免疫组化染色两组比较差异均有统计学意义(P<0.05)。结论体外冲击波疗法可促进兔激素性股骨头缺血坏死中VEGW和BMP的表达。
Objective To observe the VEGF and BMP in the glucocorticoid-induced ischemic necrosis of femoral head treated with extracorporeal shock wave therapy.Methods40 New Zealand rabbits were modeled in Yamamoto. Experimental group （group A）： the left side of the rabbits, femoral head treated with extracorporeal shock wave therapy; contrast group （group B）： the right side of the rabbits, femoral head without any treatment. After 1, 2, 4, 8, 12 weeks random eight rabbits to be put to death at a time.Both sides of all the rabbits, femoral head were observed in HE staining for cell count, the expression of VEGF and BMP immunohistochemical observation.Results HE dyeing observation after 4, 8, 12 weeks, group A blood capillary count average were 9.95±2.14, 14.70±3.08, 13.15±3.07, and group B were 4.85±1.67, 4.30±1.63, 4.40±1.79; Osteoblast count group A mean value of 33.35±3.39, 47.35±5.20, 53.40±5.09, while group B were 24.1±2.45, 21.70±4.08, 19.60±4.28, HE staining was similar between the two groups had statistical significance （P〈0.05）. Immunohistochemical staining after 2, 4, 8, 12 weeks of VEGF expression in group A mean value of 0.104±0.018, 0.177±0.020, 0.167±0.017, 0.144±0.031, while group B were 0.074±0.017, 0.078±0.021, 0.078±0.023, 0.070±0.019; And the expression of BMP group A mean value of 0.210±0.028, 0.304±0.039, 0.292±0.052, 0.268±0.053, while group B were 0.136±0.025, 0.140±0.033, 0.144±0.029, 0.118±0.028, immunohistochemical staining was similar between the two groups had statistical significance （P〈0.05）.Conclusion Extracorporeal shock wave therapy can promote rabbit VEGF and BMP in the glucocorticoid-induced ischemic necrosis of femoral head.
出处
《中华临床医师杂志（电子版）》
CAS
2014年第13期87-91,共5页
Chinese Journal of Clinicians(Electronic Edition)
基金
山西省科技攻关计划项目(20100311097)
关键词
股骨头坏死
血管内皮生长因子类
骨形态发生蛋白质类
体外冲击波疗法
Femur head necrosis
Vascular endothelial growth factors
Bone morphogenetic proteins
Extracorporeal shock wave therapy
