摘要
为选育双抗烟草花叶病毒(TMV)和马铃薯Y病毒(PVY)的烟草材料,采用Coker176与NC55杂交后代F1与非洲烟杂交获得单倍体苗,经过苗期抗性鉴定、分子标记辅助选择和叶脉培养加倍,获得母本来源的双抗TMV和PVY的双单倍体。通过人工接种抗性鉴定、标记检测和田间株系比较,获得抗性、植物学性状稳定的双单倍体第三代种子。与常规杂交系统选育相比较,双单倍体方法的获得后代变异更大、性状更容易稳定。
Doubled haploid （DH） technique can accelerate traits stabilization in hybridization offspring. In the breeding of variety resistant to Tobacco mosaic virus （TMV） and Potato virus Y （PVY）, haploids were obtained from F1 hybrids between Coker176 and NC55 crossing as female to Nicotiana african. TMV and PVY resistant haploids were selected by seedling resistance identification and molecular marker test. DH plants were regenerated from leaf mid-vein culture method. Artificial inoculated resistance evaluation, markers detection, and field comparison were conducted. The third generation seed of DH lines which showed stable resistance and botanical characters were harvested. Compared to conventional hybrid breeding methods, DH breeding method showed greater variation between offspring lines and objective traits were relatively more stable.
出处
《中国烟草学报》
EI
CAS
CSCD
北大核心
2014年第3期84-88,共5页
Acta Tabacaria Sinica
基金
中国烟草总公司云南省公司科技项目(2010YN01
2012YN02)
云南省社会发展科技计划(2010CD127)
