摘要
为了探明新疆塔城地区绵羊细粒棘球蚴流行株基因型,根据GenBank公布的12S rRNA基因和CO1基因分别设计特异性引物,对58个绵羊细粒棘球蚴包囊进行PCR检测,再通过CO1特异性引物对检测阳性病料进行PCR扩增、测序及基因分型。通过12SrRNA基因检测发现58份样品均为阳性;对所测得的58条CO1基因序列分析,发现新疆塔城地区绵羊细粒棘球蚴流行株基因型均为G1型,且CO1基因序列存在多态性,为塔城地区绵羊细粒棘球蚴病的防控提供了科学依据。
In order to understand the genotype of Echinococcus granulosus （Eg） infecting sheep in Tacheng, Xinjiang, specific primers were designed respectively according to the 12S rRNA gene sequence and CO1 gene se- quence accessed by GeneBank. 12S rRNA-specific primers were used to detect the 58 Cyst samples by PCR infected with Eg in Tacheng, then the COl-specific for PCR amplification, sequencing and genotyping for the sample tested to be positive disease. The result showed the 58 samples were all positive afier being tested by 12S rRNA gene. Analyzing the 58 CO1 gene sequences reuealed, the sheep were all infected G1 genotype of Eg in Tacheng district, Xinjiang. The comparison and analysis of Eg gene sequences showed the gene sequences were polymorphism, which can provide a scientific reference for the prevention and control of sheep Eg in Tacheng district.
出处
《家畜生态学报》
北大核心
2014年第6期65-68,共4页
Journal of Domestic Animal Ecology
基金
农业公益行业专项子课题(201303037-5)
兵团国际科技合作计划项目(2012BC006)
兵团博士资金(2010JC09)
关键词
细粒棘球蚴
基因型
绵羊
Echinococcus granulosus
genotypes sheep
