摘要
1987年从泰安表现系统花叶的扁豆上分离到一个分离物B_2,汁液摩擦接种9科38种植物,它可侵染5科10种植物,在扁豆和昆诺藜上引起系统花叶,在苋色藜上引起局部枯斑。该分离物钝化温度为60—65℃,稀释限点为10^(-3)—10^(-4),体外存活期为3—5天。可由桃蚜传播;病毒粒体线条状,大小为700—760×12nm病叶细胞内有风轮状内含体,该分离物与三叶草黄脉病毒的抗血清有明显的-阳性反应。根据这些特性,该病毒属于马铃薯丫病毒组的三叶草黄脉病毒。
An isolate of virus was obtained from Dolichos lablab in Tai-an, Shan. dong Province. It infected 10 plant species from 5 families out of 38 plant species from 9 families tested. It caused systematic mosaic on Dolichos lablab and Chenopodium quinoa, and local lesions on C. amaranticolor. Its thermal inactivation point was 60—65℃ dilution end point was 10^(-8)-10^(-4) and longevity in vitro was 3—5 days. Myzus persicae was demonstrated as aphid vector Virus particles were filamentous, about 700—760nm×12nm. Pinewheel inclusion bodies were observed in the eytoplasm of the infected leaf under electron microscope. The virus reacted positively with the antiserum of Clover Yellow Vein Virus (CYVV)by imunodiffusion tests. On the basis of these eharacteristics, the virus isolate was considered to be identical to CYVV of the Potyvirus group.
出处
《中国病毒学》
CSCD
1991年第3期223-226,共4页
Virologica Sinica