摘要
热激时,雄性不育高梁中4种酶的同工酶的变化以细胞色素氧化酶最大,酸性磷酸酯酶次之,淀粉酶第三,酯酶最小,说明不同酶对热激的反应不同,故与育性的关系也有差异。苗期与穗期同工酶谱互不对应,表明酶随发育而改变。不育系的细胞色素氧化酶酶带C5,C8随温度上升而减弱,达40℃时与可育的保持系相似,此现象与不育系的雄蕊在40℃时呈现可育态同时发生,说明此酶带与育性相关。
The induction of male sterile (M. S. ) sorghum into male fertile phenotype by heat shock (40℃) was found, which enlightened us to study the relationship of the heat shock response with male sterility. We consider this a physiological phenomenon for it did not appear in the offsprings. The isozymes of 4 enzymes were studied and compared with the male fertile maintainer line. The induction of the enzymes by heat shock was in the following order: cytochrome oxidase, acid phosphatase, amylase, and esterase. It indicated that different enzymes had different functions in affecting male sterility. The zymogram of seedlings differed from that of ears. It showed that the isozyme composition of the enzymes changed with plant development. The isozyme bands A3 and A4 of amylase and the isozyme bands C5 and C8 of cytochrome oxidase in M. S. sorghum were diminished by heat shock. When the temperature increased to 40℃, the bands C5 and C8 were similar to those of the maintainer line and disappeared with further increase of temperature, and were finally replaced by bands C7 and C9. Those isozyme bands C5 and C8 which appeared concomittantly with male fertility may have close relation with male sterility.
关键词
高粱
雄性不育
热激反应
酶
heat shock response
male sterile
isozyme
