摘要
This study was designed to compare the effect of methionine(Met)sources(DL-methionine[DLM]and DL-2-hydroxy-4-methylthio-butanoic acid(HMTBa))and their supplementation levels on broiler growth performance and redox state.A 2 x 2 factorial arrangement was used with 2 sources(DLM and HMTBa)and 2 supplementation levels(0.05% and 0.252%)of Met.A total of 480 one-day-old broiler chicks were randomly divided into 4 treatments with 8 replicates per treatment(15 birds per replicate).The experiment lasted for 21 d.Broiler growth performance,redox capacity,redox-related genes expression,and Met transporters in different tissues were tested.Broilers fed high Met supplementation levels had improved(P<0.05)body weight(BW).average daily gain(ADG)and feed conversion ratio(FCR).Similarly,broilers fed high Met levels had better(P<0.05)antioxidant abilities in the serum,small intestine,and liver.Whereas,interactive effects(P<0.05)were also observed between Met sources and levels.Compared with DLM.birds fed HMTBa diets had decreased(P<0.05)total glutathione(T-GSH)and oxidized glutathione(GSSG)contents in duodenum,ileum,and liver.Similarly,broilers fed HMTBa supplemented diets had increased(P<0.05)thioredoxin(Trx)gene expression in the duodenum and ileum,but decreased(P<0.05)glutaredoxin(Grx).glutathione reductase(CSR).and glutathione synthetase(GSS)genes expression.Furthermore,lower gene expression of Na+ and Cl-dependent neutral and cationic amino acid transporter(ATB0,+).and Na+ dependent neutral amino acid transporter(B0 AT)in the duodenum brush border,but higher gene expression of diamine acetyltransferase 1(SAT1)and Na+-independent branched-chain and aromatic amino acid transporter(LAT1)in the jejunum and ileum basement membrane along with higher expression of the proton dependent monocarboxylate transporter 1(MCT1)gene in the ileum were detected in birds fed HMTBa diets.In conclusion.DLM can be effectively used in glutathione synthesis to exert antioxidant functions,whereas HMTBa favors S-adenosylmethionine(SAM)synthesis and t
This study was designed to compare the effect of methionine（Met）sources（DL-methionine[DLM]and DL-2-hydroxy-4-methylthio-butanoic acid（HMTBa））and their supplementation levels on broiler growth performance and redox state.A 2 x 2 factorial arrangement was used with 2 sources（DLM and HMTBa）and 2 supplementation levels（0.05% and 0.252%）of Met.A total of 480 one-day-old broiler chicks were randomly divided into 4 treatments with 8 replicates per treatment（15 birds per replicate）.The experiment lasted for 21 d.Broiler growth performance,redox capacity,redox-related genes expression,and Met transporters in different tissues were tested.Broilers fed high Met supplementation levels had improved（P<0.05）body weight（BW）.average daily gain（ADG）and feed conversion ratio（FCR）.Similarly,broilers fed high Met levels had better（P<0.05）antioxidant abilities in the serum,small intestine,and liver.Whereas,interactive effects（P<0.05）were also observed between Met sources and levels.Compared with DLM.birds fed HMTBa diets had decreased（P<0.05）total glutathione（T-GSH）and oxidized glutathione（GSSG）contents in duodenum,ileum,and liver.Similarly,broilers fed HMTBa supplemented diets had increased（P<0.05）thioredoxin（Trx）gene expression in the duodenum and ileum,but decreased（P<0.05）glutaredoxin（Grx）.glutathione reductase（CSR）.and glutathione synthetase（GSS）genes expression.Furthermore,lower gene expression of Na+ and Cl-dependent neutral and cationic amino acid transporter(ATB0,+).and Na+ dependent neutral amino acid transporter（B0 AT）in the duodenum brush border,but higher gene expression of diamine acetyltransferase 1（SAT1）and Na+-independent branched-chain and aromatic amino acid transporter（LAT1）in the jejunum and ileum basement membrane along with higher expression of the proton dependent monocarboxylate transporter 1（MCT1）gene in the ileum were detected in birds fed HMTBa diets.In conclusion.DLM can be effectively used in glutathione sy
基金
supported by Beijing Technology Program[Z181100009318008]
Beijing Agricultural Innovation Consortium(BAlC04-2018)
