摘要
目的 探讨DPC4蛋白的缺失表达与胆道恶性肿瘤发病机制的关系。 方法 我们通过免疫组织化学法检测了 71例原发性胆道恶性肿瘤中DPC4蛋白的表达 ,其中包括 :38例胆总管癌 ,18例胆囊癌 ,15例肝门部胆管癌。另外 ,胆总管癌按是否伴远处转移分为转移组 (2 7例 )和非转移组 (11例 )。 结果 DPC4蛋白的缺失表达率在胆总管癌中为 4 7 3% ,在胆囊癌中为 11% ,在肝门部胆管癌中为13%。胆总管癌中的DPC4蛋白的缺失表达率与胆囊癌中的和肝门部胆管癌中的相比差异有极显著性 (P <0 0 1)。在胆总管癌转移组中DPC4蛋白的缺失表达率为 4 8 1% ,在非转移组为 4 5 4 % (P >0 0 5 )。 结论 DPC4基因的失活与胆道恶性肿瘤的发生关系密切 ,且其在不同部位的胆道恶性肿瘤中失活率差异较大 ,但与胆总管癌的远处转移关系不明显。
Objective To clarify the relationship between the loss of expression of the deleted in pancreatic carcinoma locus 4 (DPC4) proteins and the pathogenesis of biliary tract carcinoma. Methods 71 primary biliary tract carcinoma (BTCa), including 38 common bile duct (CBD) carcinomas, 18 gallbladder carcinomas, 15 hilar bile ducts (HBD) carcinomas were examined by immunohistochemical staining. In addition, the CBD carcinomas were divided into two groups: tumors with metastasis (M + group, 27 cases) and tumors without metastasis (M - group, 11 cases). Results The frequency of loss of the expression of DPC4 protein was 32 8% in BTCa, 47 3% in CBD carcinoma, 11% in gallbladder carcinoma, 13% in HBD carcinoma. Comparison of the frequency of loss expression of DPC4 was significant statistical difference in CBD carcinoma versus gallbladder carcinoma and HBD carcinoma ( P <0 01). The frequency of loss expression of DPC4 was 48 1% in the M + group and 45 4% in the M - group. Conclusion There are a close relationship between pathogenesis of BTCa and inactivation of DPC4 and different frequencies of DPC4 gene alternation in various locations of the biliary tract, which are not significantly increased with tumor metastasis in BTCa.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2002年第7期481-484,共4页
Chinese Journal of Surgery
