摘要
目的 :通过对下咽癌细胞增殖细胞核抗原 (PCNA)及其基因的检测 ,以探讨其对下咽癌生物学特性、临床行为及预后的影响。方法 :用免疫组织化学染色SABC法检测 4 8例下咽癌手术标本和同期 1 5例癌旁正常组织标本PCNA的表达 ,计算其增殖指数 ;同时用寡核苷酸探针原位杂交技术检测PCNA的mRNA ,以检验免疫组化与原位杂交结果的一致性。结果 :下咽癌组PCNA增殖指数为 (71 .4 8± 1 1 .1 4 ) % ,对照组为 (2 1 .1 3±1 2 .0 7) % ,其差异有显著性意义 (P <0 .0 5 ) ;PCNA增殖指数与癌的临床分期、组织分化程度及预后相关 ,而与是否有颈淋巴结转移和肿瘤发生部位无明显关系 ,且免疫组化和原位杂交两种检测结果相一致 (符合率为 85 .7% )。结论
Objective:To study the relationship of proliferating cell nuclear antigen (PCNA) and the biological and clinical characteristics of hypopharyngeal carcinoma and its value of clinical prognosis. Method:The SABC immunohistochemical method and in situ hybridization were correspondingly used to detect the expression of PCNA and its gene in 48 cases of cancer tissue and 15 cases of nearby cancer tissue of primary hypopharyngeal carcinoma.The number of positive cells of PCNA in 63 cases of tissue was represented by proliferating index (PI).Result:The expression of PCNA correlated with the clinical stage,histological grade and clinical recurrence of hypopharyngeal carcinoma and prognosis of the patients,but did not correlate significantly with tumor location and whether metastasis of cervical lymph nodes or not.Conclusion:The expression of PCNA protein may provide a reference in evaluating the malignancy level of hypopharyngeal carcinoma and prognosis of the patients.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2002年第7期346-348,共3页
Journal of Clinical Otorhinolaryngology
关键词
下咽肿瘤
增殖细胞核抗原
免疫组织化学
原位杂交
Hypopharyngeal neoplasms
Proliferating cell nuclear antigen
Immunohistochemtry
In situ hybridization
