摘要
目的 探讨在筋膜上培养软骨细胞修复关节软骨大面积缺损的能力和生物特性。方法 将幼兔关节软骨消化、分离和传代后的软骨细胞在兔筋膜上培养 ,分别用培养后的新鲜、冻存筋膜软骨细胞和游离软骨细胞移植修复关节软骨大面积缺损。术后 6、12及 2 4周取材 ,通过大体标本、光学显微镜、扫描、透射电镜、放射自显影以及一氧化氮含量测定等方法进行观察。结果 分离后的关节软骨细胞在兔筋膜上生长代谢良好 ,冻存后的筋膜软骨细胞生物活性无损伤 ,移植后修复的关节软骨缺损在细胞形态、生物特性与正常关节软骨组织相同。结论 筋膜可以作为软骨细胞移植较为理想的载体 ,用其修复关节软骨大面积缺损是一种有效可行的方法。
Objective To study the biological characteristic and potential of chondrocytes grafting cultured on fascia in repairing large defect of articular cartilage in rabbits. Methods Chondrocytes of young rabbits were isolated and sub cultured on fascia. The large defect of articular cartilage was repaired by grafts of freeze preserved and fresh chondrocytes cultured on fascia, and free chondrocytes respectively; the biological characteristic and metabolism were evaluated by macroscopic, histological and immunohistochemical observations, autoradiography method and the measurement of nitric oxide content 6, 12, 24 weeks after grafting. Results The chondrocytes cultured on fascia maintained normal growth feature and metabolism, and there was no damage to chondrocytes after cryopreservation; the repaired cartilage was similar to the normal cartilage in cellular morphology and biological characteristics. Conclusion Chondrocytes could be cultured normally on fascia, which could be used as an ideal carrier of chondrocytes.
出处
《中国修复重建外科杂志》
CAS
CSCD
2002年第4期223-227,共5页
Chinese Journal of Reparative and Reconstructive Surgery
