摘要
将萝芙木(Rauvolfia verticillata)的无菌苗被含有Ri-质粒的发根农杆菌(Agrobacterium rhizogenes)感染后,诱导出毛状根(hairy root)。将毛状根分离,除菌后,在不含激素的MS琼脂培养基上培养。为进一步研究萝芙木毛状根的遗传表达以及次生代谢产物的定性定量分析奠定了基础。
The sterilized plant of Rauvolfia verticillata after being infected by Agrobacterium rhizogenes were induced to produce hairy root. They grew well in medium of hormone free MS. This experiment laies a foundation for plant secondary products.
基金
国家自然科学基金
关键词
毛状根
转化
植物
次生代谢产物
hairy root
secondary metabolites proeucts
Rauvolfia verticillata
