摘要
通过对来源于 2 0~ 30年优树、5~ 8年生幼树、1~ 2年生超级苗及伐根 1~ 2年生萌生条上的顶芽、侧芽为外植体 ,对紫椴进行组织培养。采用正交试验法对基本培养基、激素种类与激素浓度进行了筛选 ,找出影响芽分化的主要因子及最佳组合试验。
Taking terminal and lateral buds as explant in superior trees at 20~30 ages,seedlings at 1~2 ages and sprout seedlings at 1~2 ages,carried out tissue culture of Tilia amurensis.Using orthogohal experiment were screened types and concentrations of minimal medium and hormone,and found out the major factors effecting on bud differentiation.
出处
《防护林科技》
2002年第2期37-38,46,共3页
Protection Forest Science and Technology
关键词
紫椴
组织培养
芽分化
培养基
激素
Tilia amurensis RuPr
tissue culture
bud differentiation