摘要
目的 观察重组人骨形态发生蛋白 2 (rhBMP 2 ) /明胶复合材料修复犬长骨节段性骨缺损的能力 ,检验rhBMP 2的诱导成骨活性。方法 手术造成 2 0mm桡骨中上段骨缺损 ,实验组植入含 2 .0mg的rhBMP 2的复合材料片 ,对照组植入单纯明胶片。通过影像学、组织学观察及骨密度测定 ,评价rhBMP 2 /明胶复合材料修复犬长骨节段性骨缺损的效果。结果 影像学检查示实验组术后 12周时骨痂将缺损桥接 ,术后 2 4周时达到皮质骨连接 ;对照组无骨痂形成。组织学检查示实验组术后 12周时骨痂外层为板层骨 ,中央为编织骨 ,内有骨髓组织 ,术后 2 4周时骨痂外层为皮质骨 ,中央为髓腔组织 ;对照组为纤维连接。骨密度测定示术后 12周时骨痂密度达到正常值的 79% ,2 4周时达到正常值的 88%。结论 rhBMP 2 /明胶复合材料具有良好的诱导成骨活性作用 ,2 .0mg的rhBMP 2能够很好的修复犬桡骨 2
Objective To survey the effect of the composite of rhBMP 2 and gelatin in repairing the segmental defect of dog's long bone, and to examine the activity of rhBMP 2.?Methods A 20mm defect was made in middle superior segment of dog's radius.In experiment group,the composite of rhBMP 2 and gelatin was implanted into the defect;?in control group only gelatin was implanted. The repairing effect was evaluated in 12 and 24 weeks after operation by radiograph, histology and BMD (bone mineral density).?Results Radiographically,all defect sites were bridged by callus by 12 weeks and connected by cortical bone by 24 weeks in experiment group. But in control group, no callus was seen. Histologically, by 12 weeks, defect sites in experiment group were bridged by lamellar bony callus, in witch there was myeloid tissue. And by 24 weeks,compact bone was seen in the outside of callus, and the medullary cavity in the centre. In control group, defect sites were connected by fibrous tissue. The BMD of the new callus was about of 79% of that of the normal radius by 12 weeks, and 88% by 24 weeks.?Conclusion The composite of rhBMP 2 and gelatin has satisfactory activity of bone induction, and 2.0mg rhBMP 2 helps repair of 20mm bone defect in dog's radius.
出处
《创伤外科杂志》
2002年第4期210-212,共3页
Journal of Traumatic Surgery
基金
国家"863"课题(218-05-28)
