摘要
目的 对分离自酵素菌的 11株酵母菌 (Yeast)进行分类鉴定。方法 通过实验观察酵母菌株的细胞形态、大小、结构 ,繁殖方式 :单极芽殖、二极芽殖、多边芽殖或裂殖。能否形成非结合子囊、结合子囊和子囊孢子。菌落类型 :光滑型、粗糙型或粘液型。菌落的大小形态、质地、颜色、透明度、气味。假菌丝生长试验 ,掷孢子试验。碳水化合物的发酵试验 ,碳源、氮源的同化试验。无维生素培养基生长试验 ,放线菌酮抗性试验 ,尿酶、卵磷、土温分解试验。重氮基蓝B试验 ,类淀粉化合物形成试验 ,5 0 %葡萄糖生长试验 ,10 %NaCl生长试验 ,37℃、40℃生长试验等项生理学和生物化学实验 ,对酵母菌的表型性状进行研究。结果 5株酵母菌鉴定为粉状毕赤酵母 ;6株酵母菌鉴定为酿酒酵母。结论 粉状毕赤酵母和酿酒酵母是构成酵素菌菌种的主要活性菌种。对分离自酵素菌的 11株酵母菌 (Yeast)进行分类鉴定。
Objective To identify eleven strains of the Yeasts from Enzymatic Microorganism.Method The following assays was done,including observation of the size,structure,characteristic of stain,types of asexual reproduction and sexual reproduction(monopolar budding,bipolar budding,multilateral budding and fission),types of ascus(non-linkascus,linkascus and ascusspore),colony(smooth colony,rough colony and mucoid colony),size,quality,colour,transparency and smell of colony,taking shape on artificial hypha and ballistoconidia,ferment experiment of compounds carbon source:glucose,galactoes,sucrose,maltose,lactose and raffinose.utilization experiment nitrogen,source:nitrate,nitrite,ethylamine HCl,cadaverine 2HCl,L-Lysine,creatine and creatinine,galactoes,Lsorbose,sucrose,maltose,cellobiose,trehalose,lactose,elibiose,raffinose,melezitose,D-xylose,L-arabinose,D-arabinose D-ribose,L-rhamnose,erthritol,ribitol,D-mannitol,inositol,glycerol,galactitol,D-sorbitol,succinic acid,citric acid,DL-lactic acid and salicin.Growth experiment in vitamin-free yeast base.Resistance experiment to the action of 100U and 1000U cycloheximide.Hytrolysis decomposition reaction on urease and tween and lecithin.reaction on DBB.Reaction took shape like-starch in test tube.Growth experiment in 50% lucose.Growth experiment in 10%NaCl.Growth experiment on 37℃ and 40℃.Nutritional need,ways of metabolism,and determination metabolism.Result Five of the yeast strains were proved to be Pichia farinosa(Lindner) Hansen and the rest six Saccharomyces cerevisiace.Conclusion Pichia farinosa(Lindner) Hansen and Saccharomyces cerevisiace are the main types of active bacterium of Enzymatic Microorganism.
出处
《潍坊医学院学报》
2002年第2期81-85,共5页
Acta Academiae Medicinae Weifang
基金
山东省科委基金资助课题 (课题号 :981 15470 3)
关键词
酵素菌
菌肥
粉状毕赤酵母
酿酒酵母
分离
鉴定
Enzymatic Microorganism
Bacterial fertilizer
Yeast
Pichia farinosa(Lindner) Hansen
Saccharomyces cerevisiace
