摘要
麻黄碱与伪麻黄碱是中药麻黄的主要有效成分,也是一对化学物理性质很相似的差向异构体。二者的结构只是构象不同,用色谱方法进行分离有一定的难度。目前已有在苯基和腈基键合固定相上进行HPLC分离的报道。本文成功地引入共轭方向法在μBondapak C_(18)色谱柱上对流动相组成进行了条件优化,优化后的色谱条件下麻黄碱与伪麻黄碱的分离情况令人满意。
The isomers ephedrine and pseudoephdrine are difficult to be separated by HPLC. In this paper, the Powell method was successfully introduced to optimize the composition of mobile phase in HPLC. On a μ Bondapak C_(18) column (8～10μm, 3.9mm×30cm), KH_2PO_4 solution methanol mobile phase system was optimized forseparating ephedrine and pseudoephedrine by the Powell method. The optimized composition of the mobile phase was found as: 0.01mol/L KH_2 PO_4—MeOH=94: 6 (V/V). The Powell method was carried out in a process illustrated by tables and figures and 0.95 peak separation function was achieved.
出处
《药学学报》
CAS
CSCD
北大核心
1991年第1期49-52,共4页
Acta Pharmaceutica Sinica
基金
国家自然科学基金
关键词
共轭方向法
优化
HPLC
麻黄碱
Powell method
Optimization
HPLC
Ephedrine
Pseudoephedrine
