摘要
目的 :探讨L -精氨酸 (L -arg)对人肾系膜细胞胞外基质产生的影响及其作用机制。方法 :运用放射免疫分析技术及羟 -脯氨酸比色法分别检测L -arg作用后系膜细胞上清液中Ⅲ型前胶原与总胶原含量 ;运用RT-PCR技术检测L -arg对人肾系膜细胞Ⅳ型胶原基因表达的影响。结果 :L -arg抑制Ⅲ型前胶原产生 ,抑制总胶原分泌 ,并抑制Ⅳ型胶原基因表达。结论 :L -arg抑制人肾系膜细胞胞外基质产生 。
AIM: To investigate the effect of L-arginine(L-arg) on the production of collagens of human mesangial cells. METHODS: Radioimmunoassay, hydroxyproline colorimetric assay and reverse transcription polymerase chain reaction (RT-PCR) were used to determine procollagen Ⅲ, total collagen level in the supernatant and expression of collagen Ⅳ mRNA in human mesangial cells. RESULTS: L-arg significantly inhibited the production of procollagenⅢ, total collagen in the supernatants ( P< 0 05, P< 0 01, respectively) and the expression of collagen IV mRNA ( P< 0 01) in human mesangial cells. CONCLUSION: This study clearly demonstrated that L-arg could exert an inhibitory effect on the production of extracellular matrix (ECM) of human mesangial cells implicating its potential therapeutic significance in treating chronic renal scarring.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2002年第5期469-472,共4页
Chinese Journal of Pathophysiology
基金
铁道部科技基金资助项目 (J97Z0 2 7)
