摘要
研究了文山松毛虫质型多角体病毒 (DpCPV W )在Sf2 1细胞中的离体增殖行为 ,并进行了空斑试验 ,结果显示DpCPV W毒株能够在Sf2 1细胞中增殖 ,也能在Sf2 1细胞上形成空斑 ,并能产生形态正常的病毒多角体。在DpCPV W中加入基因工程增效蛋白 ,能显著增加病毒粒子对离体细胞的感染率 ,增幅达 145 %。生物测定表明 ,离体增殖的病毒多角体与虫体增殖的多角体的毒力相当。因此 ,Sf2 1细胞可以作为文山松毛虫CPV增殖行为研究的离体细胞系统 。
We have studied amphification of DpCPV-W in vitro in Sf21 cell in this paper.The results showed that DpCPV-W could amplify in vitro in Sf21 cell and form normal polyhedra.Accessional rev combinant enhancin in medium could synergist the infective rate of DpCPV-W in Sf21 cell in vitro .Bioassay indicated that the toxicity of DpCPV-W polyhedra amplified in Sf21 cell was as violence as the polyhedra amplified in pine caterpillar.By using the plaque purfication technique in Sf21 cell,DpCPV-W could be purified in a single virion clone level.
出处
《中国病毒学》
CSCD
2002年第2期137-141,共5页
Virologica Sinica
