摘要
为了探讨 IFN- γ在沙眼衣原体 (chlam ydia trachomatis,ct)感染细胞培养中的影响 ,本实验采用不同浓度 γ-干扰素作用于 ct感染的 He L a细胞 ,用透射电镜观察 He L a细胞内原体 (elementary bodies,EBs)和网状体 (reticulate bodies,RBs) ,同时用反相高效液相色谱法 (reversed- phase high perform ance liquid chrom atography,HPL C)测定细胞内色氨酸的浓度。结果显示不同浓度 γ-干扰素作用下细胞内 ct的 EBs和 RBs形态及数量不同 ,中高浓度的 IFN- γ作用下胞内 EBs和RBs明显减少或轻度减少 ,ct生长受到抑制 ,细胞内色氨酸含量与 γ-干扰素量呈负相关 ,说明 IFN- γ通过诱导产生 2 ,3-吲哚 -双加氧酶 (indoleamine2 ,3- dioxygenase,IDO)降解色氨酸而抑制 ct生长。提示 γ-干扰素是抑制细胞内感染
In order to investigate the effect of IFN γ on HeLa cells by chlamydia trachomatis. The HeLa cells were treated by IFN γ in different concentration,and EBs and RBs in host cells were observed with transmission electron microscopy,meanwhile, the content of tryptophan in host cells was analysed with reversed phase high performance liquid chromatography. The result demonstrated that the numbers and forms of EBs and RBs of trachomatis chlamydia varied ,the amount of EBs and RBs was obviously or lightly decreased, and the multiplication of treachometous chlamydozoan has been inhibited there is negative correlation between the content of tryptophan and the concentration of IFN γ. It implied that IDO production induced by IFN γ can decompose tryptphan and inbibit multipliction of trachomatous chlamydozoan. These findings provide that IFN γ may play a critical role in inhibition of the multiplication of trachomatous chlamydozoan in infected cells.\;
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2001年第2期182-185,共4页
Chinese Journal of Histochemistry and Cytochemistry
