摘要
植物抗虫基因工程是解决农业生产中虫害问题的一种重要手段,利用抗虫基因使植物获得抗虫性已经得到了实际应用.在可以利用的抗虫基因中,植物外源凝集素基因是一个重要方面.植物外源凝集素是一组广泛存在于植物组织中的蛋白质成分,现已发现多种植物外源凝集素对同翅目、鞘翅目、鳞翅目害虫有抗杀作用.菊芋外源凝集素是一种新发现的植物外源凝集素,本文将其置于 CaMV35S组成型启动子和Tnos终止子的控制下,构建了用于转化烟草的植物表达载体,转化烟草并获得了转基因烟草,以期下一步对菊芋外源凝集素的抗虫性做出评估.
Insect-resistant plant genetic engineering is a way to overcome the disturbing problem of pests in agri-cliture and plant agglutinin genes are of great importance in the aspect of insect-resistant genetic engineering a-mong the insect-resistant genes used. Plant agglutinins are carbohydrarte-binding proteins which have been found in a wide vareity of species encompassing almost every major classification of flowering plants and distributed abundantly in organs such as roots, leaves, rhizomes, and stems. Plant lectins have been showned to be insecticidal to different kinds of pests. Helanthus Tuberosus Agglutinin(HTA) is a recently found lecint whose insecticidal aspect has not been under investigation. Plant expression vector pCNHTA was constructed in which the expression of HTA was under the control of CaMV35S promoter and T-nos terminator. Tabacco was transfromed with the HTA by Agrobacterium tumefaciens LBA4404 hrboring the expressing vector pCNHTA and transgenic plants were obatained which had been confirmed by PCR analysis. The next goal will be the alalysis of resistant ability of HTA to insects.
出处
《商丘师范学院学报》
CAS
2002年第2期111-114,共4页
Journal of Shangqiu Normal University
关键词
转菊芋外源凝集素
基因
烟草
抗虫基因工程
表达载体
转化
insect-resistant plant genetic engineering
Helanthus Tuberosus Agglutinin (HTA)
expression vector
transformation
