摘要
采用血涂片、Feulgen染色和显微分光光度技术 ,测定了金线属 (Sinocyclocheilus) 17个种的核DNA含量。结果显示 ,除侧条金线 (S .lateristritus)中采自云南沾益炎方山那边的样本之 2C值为 7 79pg外 ,其余的核DNA 2C值都集中分布在 4 19～ 4 86pg范围 ,大体与其近缘四倍体种 2C值相同或相近 ,是其近缘二倍体种 2C值的大约 2倍。据此我们推断 ,这 17种金线很可能都是四倍体 ,个别种还含有八倍体的类型 ,金线属可能是整属的四倍体起源。
The genus Sinocyclocheilus (Cypriniformes: Cyprinidae: Barbinae) is endemic to China and only found in Yunnan, Guizhou and Guangxi Provinces. The nuclear DNA content of two species and the ploidy level of three species in Sinocyclocheilus had been determined in the middle of 1980s. In recent years, more and more new species were reported in this genus. Therefore, it is necessary to analyze the nuclear DNA content, ploidy level and evolution of the species in the genus. In the present paper, by means of the red cell spreading-Feulgen staining-cytophotometry technique, we have measured the nuclear DNA content of 17 species in Sinocyclocheilus from Yunnan, Guizhou and Guangxi Provinces and distributed in the drainage of Nanpanjiang, Jinshajiang, Beipanjing, Yuanjiang, Hongshuihe and Youjiang rivers. The results showed that the nuclear DNA content of all 17 species were between 4 19 and 4 86?pg, as the same level of the related tetraploid species, doubled that of the related diploid species. It is likely that all of these 17 species and the whole genus Sinocyclocheilus have tetraploid origin.
基金
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关键词
金线BA
核DNA含量
倍性
Sinocyclocheilus
Nuclear DNA content
Ploidy
Origin
