摘要
目的 :了解B细胞淋巴瘤白血病 (LL)患者TCRVβ亚家族T细胞的分布及其克隆性。方法 :利用RT -PCR分别扩增 3例LL患者外周血单个核细胞的TCRVβ 2 4个亚家族基因的CDR3,以了解患者各Vβ亚家族的利用情况 ,然后将阳性的PCR产物进一步经荧光素标记和基因扫描分析产物的CDR3长度 ,了解T细胞克隆性。结果 :患者中仅存在 2～ 6个Vβ亚家族T细胞。基因扫描分析显示 ,2例患者外周血中的Vβ1、Vβ3或Vβ4亚家族出现克隆性增殖T细胞。结论 :LL患者外周血存在倾斜性分布和克隆性增殖的Vβ亚家族T细胞 ,这可能是机体T细胞受白血病细胞相关抗原的刺激作用而引起机体产生相应的特异性抗白血病的免疫反应。
Aim: To investigate the distribution and clonal expansion of TCR Vβ subfamily T cells in patients with B cell lymphoma leukemia (LL) . Methods: The CDR3 of TCR Vβ 24 subfamily genes were amplified in peripheral blood mononuclear cells from 3 cases with LL using RT-PCR, to observe the usage of TCR Vβrepertoire, the PCR products were further labeled with fluorescent and analyzed by genescan technique for the CDR3 size, to evaluating clonality of the detectable TCR Vβ T cells. Results: Only 2-6 Vβ subfamily T cells could be identified in the patients with LL, and oligoclonal (clonal expansion) T cells could be found in Vβ1, Vβ3 or Vβ4 subfamilies from 2 cases. Conclusion: The skew distribution and clonal expansion of TCR Vβ subfamily T cells could be found on patients with LL. It may be a specific anti-leukemia immune response that host T cells activate by the leukemia-associated-antigen.
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
2002年第2期7-10,共4页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
国家自然科学基金 (39870 35 8)
广东省自然科学基金 (980 70 5 )
广州市科委重点科技攻关计划 (98-z - 0 39-0 1)
广东省医学科研基金 (A19992 71)资助项目
