摘要
目的 :了解不同分子量透明质酸对人腹膜间皮细胞透明质酸合成酶 (HAS)的调节作用及对透明质酸合成的影响。方法 :分离培养的人腹膜间皮细胞 (HPMCs)随机分为 3组 :正常对照组 (对照组 ) ;高分子量透明质酸组(分子量为 1,6 0 0 ,0 0 0道尔顿 ,浓度 0 .0 1%,HHA组 ) ;低分子量透明质酸组 (分子量为 2 80 ,0 0 0道尔顿 ,浓度 0 .0 1%,LHA组 )。给予上述刺激后继续培养 2 4h后 ,以RT -PCR法检测HAS - 2mRNA和HAS - 3mRNA的表达 ;微粒排除法观察细胞基质的面积。结果 :LHA组HAS - 2mRNA及HAS - 3mRNA表达均较对照组增强 (P值均 <0 .0 5 ) ;HHA组HAS - 2mRNA表达较对照组增强 ,而HAS - 3mRNA表达与对照组无显著差异。各组细胞胞衣样结构面积与细胞体面积的比值的比较 ,HHA组较对照组有增加趋势 ,但未达统计学差异 (P >0 .0 5 ) ,LHA组与对照组间无显著性差异。结论 :LHA和HHA均促进HPMCsHAS - 2mRNA表达 ,LHA对与炎症反应相关的HAS - 3mRNA表达也有增强作用 ,而HHA则没有这一作用。因此 ,从选择腹膜保护剂的角度分析 ,我们倾向于选择更具生物相容性的HMW -HA。
Objective:To understand the effect of hyaluronan with different molecular weights on hyaluronan synthesis in human peritoneal mesothelial cells(HPMCs).Methods:Cultured HPMCs were stimulated with 0.01% low molecular weight hyaluronan(280,000 Da,LHA)and high molecular weight hyaluronan(1,600,000 Da,HHA)respectively.Extracellular coats were observed using particle exclusion assay and the expression of hyaluronan synthase 2 and 3(HAS-2 and HAS-3)mRNA in HPMCs were measured by reverse transcription-polymerase chain reaction(RT-PCR).Results:Both LHA and HHA stimulated HAS-2 mRNA expression and only LHA enhanced HAS-3 mRNA expression.Although extracellular coats in HHA group tended to increase,the sizes of the extracelluar coats in both groups were not significantly different from that of the control group.Conclusions:Low molecular weight hyaluronan enhanced HAS-3 mRNA expression which is related to inflammation reaction.On the contrary,high molecular weight hyaluronan did not have such effect.These results suggests that high molecular weight hyaluronan may be a better additive for peritoneal dialysis.
出处
《中国中西医结合肾病杂志》
2002年第6期320-322,331,共4页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
卫生部临床学科重点基金资助项目 (No .970 40 2 2 8)
中山医科大学"2 11"工程资助项目 (No.9815 1)
瑞典KarolinskaInsti tute合作基金资助项目
