摘要
从柚子 (Citrus grandis Osbeck) 2个自交不亲和品种溪蜜柚和度尾蜜柚的花柱中克隆出一个类似 S核酸酶基因 Cg SL1(C.grandis S- like RNase) ,它的 c DNA序列全长 10 74 bp,编码 2 97个氨基酸。通过与其它植物S- like核酸酶和 S核酸酶氨基酸序列进行比较 ,发现 Cg SL1类似于 S- like核酸酶 ,与拟南芥中的 RNS2一致性为6 2 .5 %。对 Cg SL1的表达分析表明该基因在花柱、花药、叶片不同器官以及花柱的不同发育阶段均有表达 ,且在花柱中的表达随衰老增强 ,由此推测它可能与衰老有关。
A gene encoding a S like RNase (CgSL1) was isolated from the styles of two self incompatible pomelo cultivars Citrus grandis Osbeck vs.Guanxi and vs.Duwei.The cDNA of CgSL1 was 1074 bp in length with a capability of encoding a polypeptide consisting of 297 amino acids.Sequence comparison showed that CgSL1 was more similar to S like RNases than to S RNases and had a highest similarity with 62 5% identity to RNS2 from Arabidopsis thaliana.To reveal the tissue specificity and the temporal expression patterns of CgSL1,RT PCR was conducted with RNAs from several tissues.Results showed that CgSL1 expressed in styles,anthers and leaves,and its expression in styles increased in the proceeding of style senescence,suggesting that CgSL1 may be related to senescence.
出处
《武汉植物学研究》
CAS
CSCD
2002年第2期83-88,共6页
Journal of Wuhan Botanical Research
