摘要
目的 观察烧伤小鼠 2 4h内肝组织糖皮质激素受体在蛋白及mRNA水平的时相变化特点。方法 将 6 0只BALB/C小鼠随机分为正常对照组和实验组 ,实验组给予背部Ⅲ度 15 %～ 2 0 %烧伤。分别于伤后 2、4、6、12、2 4h测定血清皮质酮浓度 ,用Westernblotting、RT -PCR在蛋白质和mRNA水平测定肝组织糖皮质激素受体。结果 烧伤小鼠血清皮质酮浓度 2～ 4h达到高峰 ,2 4h仍未恢复正常 ;肝组织糖皮质激素受体在蛋白质水平 4～ 6h降至最低 ;而mRNA水平仅仅在伤后 2h有短暂降低。结论 烧伤应激引起小鼠血清皮质酮水平明显升高 ,其受体在蛋白及mRNA水平均可降低 ,但蛋白的降低不仅仅发生在mRNA水平。
Objective To observe the changes of hepatic glucocorticoid receptor(GR)at protein and mRNA level in burned mice.Methods 60 BALB/C mice were divided randomly into control and burn group.After clipping of the hair,15%～20% thickness third degree burn injury was produced.At 2,4,6,12,24 h after burn,serum corticosterone concentration was examined,hepatic GR and GR mRNA were detected by Westernboltting and RT-PCR.Results After burn injury,serum corticosterone level was markedly elevated at 2～4 h and hepatic GR was markedly downregulated at 4～6 h.The reduced GR mRNA was only observed at 2 h.Conclusions Serum corticosterone level was markedly elevated and hepatic GR was downregulated at protein and mRNA level.It is not principal machanism about downregulation of GR at mRNA lever.
出处
《中国急救医学》
CAS
CSCD
北大核心
2002年第6期311-313,共3页
Chinese Journal of Critical Care Medicine
基金
国家重点基础研究发展规划项目 ("973"项目 ) (G19990 5 42 0 0 )
