摘要
氨对重组中国仓鼠卵巢 ( CHO)细胞的生长具有明显的抑制作用 ,并遵循二级抑制模型 ,抑制常数 Ka 为 41 .5 ( mmol/L ) 2 ,即当氨浓度为 6.45 mmol/L时 ,细胞的比生长速率下降到最大比生长速率的 5 0 %。在重组 CHO细胞的批培养过程中 ,细胞密度和红细胞生成素浓度随着起始氨浓度的提高而下降 ,但存在一最适的氨浓度 ,在此浓度下 ,单位重组 CHO细胞的
The effects of ammonia on the growth of recombinant CHO cells and the expression of EPO were studied. The inhibition of ammonia over the growth of recombinant CHO cells follows the second order inhibition model, and the inhibition constant K a is 41.5(mmol/L) 2, i.e. the specific growth rate of cells reduced to the half of its maximum value at 6.45 mmol/L ammonia. It was shown that the cell density of recombinant CHO cells and EPO concentration in batch cultures with different initial ammonia concentrations reduced with the increase of ammonia concentrations. The specific production rate of EPO at 3.22 mmol/L ammonia was higher than those at others, showing that there was an optimum ammonia concentration at which the capacity of recombinant CHO cells expressing EPO was on the peak. These results may be helpful to optimize the production of EPO in the culture of recombinant CHO cells.
出处
《华东理工大学学报（自然科学版）》
CAS
CSCD
北大核心
2002年第2期172-175,共4页
Journal of East China University of Science and Technology
