摘要
通过单因子试验，研究了椭圆小球藻Ｌ１藻株异养培养发酵的最佳条件分别为：葡萄糖１０ｇ／Ｌ，尿素０．２－１ｇ／Ｌ的ＢＧ－１１培养基，培养温度２５℃～３０℃，培养基起始ｐＨ为５．５～６．５，２５０ｍＬ三角瓶装液量为１００ｍＬ，接种量１０％。在此基础上，进行了２Ｌ发酵罐培养实验，获得了干重５．４２ｇ／Ｌ的生物量，约为自养培养获得生物量的２０倍。对异养与自养藻细胞内主要营养成分的分析结果表明：异养小球藻总脂含量升高，蛋白质及灰分含量下降。
The optimal condition of Chlorella ellipsoidea L1 strain under heterotrophic culture to get high biomass were: BG-11 medium with 10g/L of glucose as the carbon source and 0.2-1g/L of Urea as the nitrogen source,25～30℃ cultivation temperature, initial pH 5.5～6.5, 100ml medium in 250ml flask and seeding 10%(V/V). By inoculating the pre-culture to a conventional 2L aerated and stirred the minijar ferment containing 1.5L of the medium, we got dry cell weight 5.42g/L. The productivity was about 20 times as much as that for photoautotrophically grown cultures. Compared with phototrophic, the lipids content increased, whereas the protein contents and ashes decreased.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2002年第4期28-33,共6页
Food Science
基金
江苏省教育委员会自然科学基金资助项目(98KJD180002)
关键词
小球藻
异养培养
营养成分
培养条件
Heterotrophic cultivation Autotrophic cultivation Chlorella ellipsoidea
