摘要
目的 研制用于Rh(D)分型的抗 D单克隆抗体。方法 以EB病毒 (EBV)转化能分泌抗 D抗体的Rh阴性供者的B淋巴细胞 ,用酶处理RhD+ 、O型红细胞富集抗 D分泌细胞 ,并用有限稀释法克隆。采用一步酶法 ,于 96孔微量板中分别用阳性细胞培养上清液和人源多克隆抗 D血清及进口的单克隆抗 D血清平行检测 10 0 0名献血员的Rh(D)血型。结果 建立了 3株能分泌抗 D抗体的人B淋巴细胞株 ,其中 1株分泌的抗体为lgM ,2株为lgG ;抗体效价在 6 4～ 12 8之间 ;3种试剂检测Rh(D)血型的符合率为 10 0 % ,3名献血员均为RhD-。结论 抗 D单克隆抗体可用于Rh(D)分型。
Objective Study on producing human monoclonal antibodies against Rhesus (D) antigen that was suitable for use as blood group typing reagent. Methods B lymphocytes from a Rh negative woman, which can produce anti D antibodies were transformed by Epstein Barr virus(EBV). Antibody secreting cells were enriched by RhD + group O erythrocytes and cloned by limited dilute method. By using one step emzymatic method on microplates, one thousand normal blood donors with a common Rh phenotype were tested with the supernatant of cell culture medium as well as a polyclonal human anti D and a commercial monoclonal anti D serum. Results Three human B lymphocyte lines secreting monoclonal antibodies to Rh (D) were established. One of them produced lgM antibody. The titer of the monoclonal antibodies was 64～128. Study on 1000 blood donors, the results did not show any discrepancy among the three different anti Rh(D) serum. Conclusion These monoclonal antibodies against D antigen could be used in Rh(D) typing.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2002年第3期166-168,共3页
Chinese Journal of Laboratory Medicine
