摘要
目的 不同病期急性白血病 (AL)患者NM2 3 H1基因mRNA表达与临床的关系。方法采用半定量逆转录 聚合酶链反应 (RT PCR)检测 82例AL患者 ,其中初诊 5 6例 ,首次缓解期 (CR) 12例 ,缓解 6个月 8例 ,复发 6例及 15例正常对照骨髓细胞NM2 3 H1和三磷酸甘油醛脱氢酶 (GAPDH)表达。将NM2 3 H1/GAPDH≥ 0 5定为阳性。结果 正常骨髓细胞NM2 3 H1表达阴性 ,5 6例初诊AL中 ,阳性表达 4 3例 ,表达范围为 0 33～ 2 75 (阳性率 76 8% ) ,CR者表达范围为 0～ 0 6 3(8 3% ) ,缓解>6个月者表达为 0～ 0 2 7(0 % ) ,复发患者表达范围为 0 76～ 1 87(10 0 % )。初诊AL患者与复发者较正常标本显著增高 (u分别为 5 4 3和 4 14 ,P <0 0 1)。NM2 3 H1mRNA水平在初诊AL中显著增高 ,NM2 3 H1表达阳性者的CR率明显低于表达阴性者 (分别为 5 0 %和 90 % ,P <0 0 1)。结论 NM2 3 H1mRNA在不同病期表达水平不同 ,高表达与白血病化疗效果相关 ,NM2 3
Objective To determine the expression of NM23 H 1 gene in acute leukemia(AL) and evaluate the relationship between NM23 H 1 expression and clinical features Methods Expression level of NM23 H 1 mRNA in bone marrow cells was determined in 82 acute leukemia patients and 15 normal subjects with semi quantitative reverse transcription polymerase chain reaction(RT PCR) Results NM23 H 1/ GAPDH ratio ≥0 5 was considered to be positive NM23 H 1 mRNA was negative in all the 15 normal subjects Expression of NM23 H 1 was positive in 43 of the 56 acute leukemia patients in the first visit, expression range being 0 33～2 75 There was one positive case in 12 AL patients with complete remission, expression range being 0～0 63,but there was no positive case in 6 AL patients who had maintained complete remission for more than 6 months, expression range being 0～0 27 Relapsed cases were all positive with an expression range of 0 76～1 87 NM23 H 1 expression in patients with initial and relapsed acute leukemia was higher than that in normal subjects ( P <0 01) Conclusion Overexpression of NM23 H 1 mRNA can predict treatment outcome and may be an important prognostic factor
出处
《中华内科杂志》
CAS
CSCD
北大核心
2002年第6期367-369,共3页
Chinese Journal of Internal Medicine
