摘要
用凤尾丝兰(Yucca gloriosa L.)离体子房为外植体接种材料,在附加KT、2,4-D及LH的MS培养基上诱导子房膨胀及愈伤组织发生;在附加6-BA、NAA及LH的MS培养基上诱导大量丛生芽;转至MS附加IBA的培养基上则能诱导根系形成;在间歇性高温灭菌及药物拌土的基质上,移植成活率达72%以上,得到了批量成苗。经镜检,试管苗与原株根蘖苗染色体数目一致。并对培养中各分化阶段进行了过氧化物酶活性测定,发现过氧化物酶活性随分化进展逐渐提高,呈正相关。
The ovaries in vitro of Yucca gloriosa were used as the explant inocalate material in order to induce ovaries to expand and callus to form in MS medium supplemented with KT, 2,4-D, and LH. A large number of thickly growing buds were sprouted in MS medium supplemented with 6-BA, NAA and LH, and then transplanted to MS medium supplemented with IBA so as to induce rootlets to form. The transplant survival rate reached more than 72% in soil medium sterilized by intermittent high temperature and mixed with germicide. A lot of complete plantlets had been obtained. Observing under microscope, the chromosome number of test tube plantlets is the same with that of original root sprouts. The peroxidase activity of every differentiation periods in culture is determined. The results show that the activity has a positive relationship with differentiation progress.
出处
《武汉植物学研究》
CSCD
1991年第3期268-274,共7页
Journal of Wuhan Botanical Research
关键词
凤尾丝兰
子房培养
器官发生
Yucca gloriosa
Ovary
In vitro culture
Peroxidase activity
