摘要
目的和方法 :采用大鼠四血管闭塞全脑缺血模型 (4 vesselocclusion ,4VO)及组织病理学方法 ,观察预缺血的持续时间 ,和预缺血与其后的损伤性缺血之间的间隔时间对海马缺血耐受形成的影响。结果 :缺血 6min即可导致海马组织明显的神经元延迟性死亡 (delayedneurondeath ,DND) ,而缺血 3min不足以引起海马组织明显的DND。经过 3min缺血预处理 ,可对间隔 1d和 3d后 6min缺血引起的大鼠海马DND产生明显的保护作用 (P <0 .0 1)。但是 ,1min缺血预处理对间隔 1d后 6min缺血引起的DND不产生明显影响 ;5min缺血预处理对间隔 1d后 6min缺血 ,以及 3min缺血预处理对间隔 1h后 6min缺血引起的DND不但没有保护作用 ,反而有使海马组织损伤累积加重的趋势。结论 :在 4VO大鼠模型中 ,全脑缺血预处理确能诱导海马对缺血性损伤产生耐受 ,诱导海马缺血耐受所需缺血预处理的适宜期间为 3min左右 ,预缺血与后续损伤性缺血之间需要间隔足够的时间 ,适宜间隔在 1～3d左右。
Aim and Methods: To investigate the role of the duration of ischemic preconditioning and the interval between the ischemic preconditioning and the following injured ischemia in induction of ischemic tolerance of the hippocampus,using 4 vessel occlusion (4VO)global ischemic model of rats. Results: Ischemia for 6 min resulted in a apparent delayed neuron death (DND) in the hippocampus, while ischemia for 3 min did not cause DND in the hippocampus. Ischemic preconditioning for 3 min could apparently decrease DND caused by ischemia for 6 min followed the preconditioning at intervals of reperfusion 1 or 3 d ( P <0.01), indicating the protective effect of the ischemic preconditioning against the following severe ischemia. However, ischemic preconditioning for 1 min did not produce any apparent protective effect. The DND could not be decreased not only, but increased in a condition of ischemic preconditioning for 5 min followed by 6 min injured ischemia at a interval of 1 d or ischemic preconditioning for 3 min followed by ischemia for 6 min at a interval of 1 h. Conclusion: Global ischemic preconditioning could induce tolerance of the hippocampus to ischemic injury. The proper duration of ischemic preconditioning and the interval between the preconditioning and the following injured ischemia might be 3 min and 1 to 3 days, respectively, in the 4VO rat model.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2002年第2期109-113,共5页
Chinese Journal of Applied Physiology
基金
河北省自然科学基金资助项目 (3 993 66)
