摘要
以三倍体毛白杨试管苗叶片作为外植体进行愈伤组织的诱导 ,最适的愈伤组织诱导培养基为 MS+ NAA1 .0 mg/L + 6- BA0 .1 mg/L;分化培养基为 :1 /2 MS+ BA0 .5 mg/L。利用腋芽增殖试管苗 ,单芽增殖有效苗可达 1 2株以上 ;生根培养基为 MS+ IBA0 .8～ 1 .2 mg/L,试管苗的生根率可达 85 .93% ;炼苗成活率达到 93.8% ,田间移栽成活率为 87.5 %。
Inducing calli from leaves of Populus tomentosa The results showed that the inducing medium of the calli is MS+NAA1.0 mg/L +6 BA0.1 mg/L and the differentiation medium is 1/2MS+BA0.5 mg/L. The twelve effective seedlings can be generated from single axillary bud. The medium for rooting is MS+IBA0.8~1.2 mg/L. Replanting survival rate is 87.5%.
出处
《甘肃林业科技》
2002年第1期5-7,共3页
Journal of Gansu Forestry Science and Technology
关键词
毛白杨
三倍体
愈伤组织
腋芽增殖
白杨树
Populus tomentosa
triploid
callus
axillary bud multiplication
