摘要
主要外膜蛋白在鹦鹉热衣原体感染过程中起主要作用。扩增了主要外膜蛋白基因 ,克隆入 pGEM T和pET32a(+) ,经PCR筛选和酶切鉴定 ,进行诱导表达和重组蛋白的纯化与复性研究 。
Major outer membrane protein plays a key role in the infection of Chlamydia psittaci.In this report,the gene of the major outer membrane protein was amplified and cloned into plasmid pGEM T and pET32a(+).The positive strains were determined by PCR and enzymic digestion and then induced.The purifiation and renaturation were further carried out to the purpose of application of the recombinant protein.
出处
《微生物学免疫学进展》
2002年第2期32-34,共3页
Progress In Microbiology and Immunology
关键词
鹦鹉热衣原体
主要外膜蛋白
克隆
Chlamydia psittaci
Major outer membrane protein
Cloning.
