摘要
目的 :建立高效毛细管电泳法测定麻仁丸和除湿白带丸中芍药苷的含量方法。方法 :运行缓冲液 :5 0mmol·L-1硼砂缓冲液 (pH10 .6 ) ;操作电压 2 0kV ;检测波长 2 30nm。结果 :测定芍药苷的线性范围为 0 .0 6 2 5～ 1.0mg·ml-1,r =0 .9995 ;麻仁丸和除湿白带丸加样回收率分别为 98.1%和 99.4 %。日内和日间精密度分别为 1.6 %和 3.2 %。测定了麻仁丸和除湿白带丸中芍药苷的含量。结论 :该分析方法简便 ,快速 ,经济 ,准确。
OBJECTIVE An high performance capillary electrophoresis (HPCE) method for determination of paeoniflorin in Maren Wan and Chushi Baidai Wan was deleloped.METHODS Measurements were carried out in a buffer solution containing 50 mmol·L -1 borax solution (pH 10.6 ),at 20 kV running voltage applied to a 50 cm×50 μm capillary.The detection wavelength was 230 nm.RESULTS The linear range of paeoniflorin was 0.0625 ～ 1.0 mg·ml -1 ,RSD within days was 1.6% ,RSD between days was 3.2% . The recovery of paeoniflorin for Maren Wan and Chushi Baidai Wan was 98.1% and 99.2% ,respectively.We also used the method to determine Maren Wan and Chushi Baidai Wan .CONCLUSIONS The method is simple,rapid,economic and accurate.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2002年第5期280-281,共2页
Chinese Journal of Hospital Pharmacy
