摘要
目的 对浓蜜贝母 Fritillaria mellea原种鳞茎进行组织培养 ,比较浓蜜贝母愈伤组织生长过程中不同时期的总生物碱含量。方法 采用不同激素、温度、光照等方法 ,探索浓蜜贝母愈伤组织的最佳诱导率条件 ,并对其总生物碱含量进行测定。结果 浓蜜贝母的最佳诱导条件为激素及浓度 :NAA 1m g/L +6 BA 6 m g/L ;温度 (2 2± 1)℃ ;散射光照 ;愈伤组织形成后 2 0 d左右时的总生物碱含量最高。结论 浓蜜贝母愈伤组织的总生物碱含量高于原种鳞茎。
Object To compare the contents of alkaloids of callus at different period with tissue culture of fresh bulbs of Fritillaria mellea S. Y. Tang et S. C. Yueh.Methods To study the best condition of induction frequency of callus about some phytohormones, temperature and light, and then to determine the contents of alkaloids of callus in F. mellea. Results The best condition of induction frequency of callus above was NAA 1 mg/L+ 6BA 3 mg/L; temperature at (22±1) ℃ and scattered light; the contents of alkaloids were kept highest in about 20th day ofter the callus appeared.Conclusion The contents of alkaloids of callus are kept higher level than fresh bulbs of F. mellea.
出处
《中草药》
CAS
CSCD
北大核心
2002年第5期458-459,共2页
Chinese Traditional and Herbal Drugs
关键词
浓密贝母
组织培养
愈伤
诱导
生物碱
中药材
采集
鉴定
Fritillaria mellea S. Y. Tang et S. C. Yueh
tissue culture
callus
induction
alkaloid
