摘要
目的 观察硫代修饰型c-myc反义寡核苷酸直接转染粘液表皮样癌MEC-1细胞后在细胞内的分布,为反义寡核苷酸抑制粘液表皮样癌细胞增殖和抑制基因表达提供形态学依据。方法FITC标记硫代磷酸化修饰的c-myc反义寡核苷酸,转染培养的粘液表皮样癌MEC-1细胞,在激光共聚焦显微镜下观察转染细胞及细胞内的时相分布。结果 硫代修饰型c-myc反义寡核苷酸直接转染粘液表皮样癌MEC-1细胞后12h,细胞胞浆内即有较强的荧光分布;转染MEC-1细胞24h细胞胞浆荧光达高峰;转染48h细胞胞浆荧光减弱;转染72h细胞胞浆荧光进一步减弱,但未消失。结论 硫代修饰型c-myc反义寡核苷酸主要分布于细胞胞浆,其作用可能是通过阻断mRNA的翻译而实现。
ve In order to provide morphological evidence, the distribution of phosphothioated (phosphoroth-ioate) c-myc antisense oligonucleotide in MEC-1 cell of mucoepidermoid carcinoma was observed. Methods Phosphothioated c-myc antisense oligonucleotide was labeled with fluorescent 5'-isothiocyananatee (5'-FITC), and then added into MEC-1 cell culture media. The intracellular distribution of fluorescence was observed by laser scanning confocal microscope. Results The fluorescent distribution in MEC-1 cellular cytoplasm gradually increased within 12 hours and came to the peak in 24 hours and gradually decreased from 48 hours after adding. Conclusion Phosphothioated c-myc antisense oligonucleotide which might act by blocking translation of mRNA was mainly distributed in cytoplasm.
出处
《口腔颌面外科杂志》
CAS
2002年第1期26-28,共3页
Journal of Oral and Maxillofacial Surgery
