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3月龄大鼠不同大动脉光镜和CLSM观察及定量分析

LM and CLSM Observation and Quantitative Analysis of Different Large Arteries in Rats of Three Months Old
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摘要 采取常规石蜡切片和 HE染色法制成切片标本 ,在光镜和激光共聚焦扫描显微镜 ( CLSM)下测量升主动脉、胸主动脉、腹主动脉和股动脉段的管壁中膜厚度、管腔面积、管壁中膜面积、弹性膜层数和弹性膜荧光强度等 .结果表明了由升主动脉至股动脉呈现血管壁中膜变薄、管腔缩小、弹性膜荧光强度降低等变化规律 . In this experiment,conventional hematoxylin-eosin staining was used to make sections,then with light microscope and confocal laser scanning microscope the changes of different elastic arteries were investigated and measured.The results are as follows:from ascending aoerta to femoral artery,both the area and the thickness of tunica media of vascular wall were obviously decreased,so was the area of vascular cavity;while the ratio of vascular wall area to vascular cativity area was obviously increased;fluorescence intensity of elastic membranes showed a tendency to decrease.In a word,great changes take place in different elastic arteries.
出处 《河北师范大学学报(自然科学版)》 CAS 2002年第3期297-300,共4页 Journal of Hebei Normal University:Natural Science
关键词 3月龄大鼠 大动脉 CLSM观察 定量分析 弹性膜 弹性动脉 光镜观察 膜厚度 管腔面积 显微结构 elastic membrane confocal laser scanning microscope elastic artery SD rat
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