摘要
本文介绍了一种膜片钳微电极内插管进行胞内透析的方法。利用通用的微电极夹持器在其抽吸负压的侧管上方钻一斜孔直通夹持器中央管腔。插入由微量移液管头拉制成的细管 (外径约 0 1mm) ,后者与Ag AgCl电极一起伸出夹持器口端。通过相连的注射器 ,可以很方便地进行电极内液置换及胞内药物透析。此法和二次钳压技术及国外介绍的微插管电极内液置换法相比 ,更加简便易行 。
In this paper we present an easily available method of intracellular dialysis via a microcatheter inserted into glass pipette during patch clamp experiment. An oblique hole through the glass pipette holder (above the lateral hole for cell seal suction) is drilled, through which a microcatheter (O.D.=0 1 mm) made from the universal pipetter tip by hand drawing passes and sticks out of the holder mouth in parallel with the Ag AgCl electrode. With a syringe connected to the microcatheter, substitution of intracellular solution and intracellular dialysis of drugs can be achieved easily. Compared with repatch technique and intracellular solution substitution techniques used abroad, this method operates more easily and can produce more reliable results.
出处
《生理学报》
CAS
CSCD
北大核心
2002年第2期179-182,共4页
Acta Physiologica Sinica
基金
ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina (No .3 9870 2 5 9)
关键词
膜片钳
微插管
微电极夹持器
电极内液置换
胞内透析
patch clamp
microcatheter
pipette holder
substitution of intracellular solution
intracellular dialysis
