摘要
目的 :研究己酮可可碱对血吸虫病肝纤维化小鼠血清一氧化氮及肝内诱导型一氧化氮合酶的影响。方法 :制备血吸虫病肝纤维化小鼠模型 ,应用免疫组化染色方法和多媒体彩色病理图文分析系统 ,定量观察不同剂量己酮可可碱治疗前后肝脏诱导型一氧化氮合酶含量的变化 ,并通过化学还原反应检测血清一氧化氮的水平变化。结果 :应用己酮可可碱治疗后可降低肝内诱导型一氧化氮合酶和血清一氧化氮的含量 ,此作用与治疗剂量有关 ,高剂量治疗组与感染组比较 ,差异有非常显著性 (P <0 .0 1) ;低剂量组与感染组及高剂量组与低剂量组间比较差异亦均具有显著性 (P <0 .0 5 )。结论 :己酮可可碱可显著降低血吸虫病肝纤维化小鼠肝脏诱导型一氧化氮合酶活性及血清一氧化氮含量 ,发挥抗血吸虫病肝纤维化的作用 ,此作用与用药剂量有关。
Objective:To study the effects of pentoxifylline on the serum level of nitric oxide and hepatic content of induced nitric synthetase in schistosomiasis mice with liver fibrosis.Method:Chemical reaction and immunohistochemical technique were applied to observe the serum level of nitric oxide (NO) and hepatic content of induced nitric oxide synthetase (iNOS) in schistosomiasis mice with liver fibrosis before and after pentoxifylline treatment. Results:It was found that pentoxifylline could reduce the content of hepatic iNOS and serum NO in schistosomiasis mice with liver fibrosis significantly,with statistical significance between high dose group and low dose group ( P < 0.05 ). Conclusion:The results showed that pentoxifylline could reduce the content hepatic iNOS and serum NO in a dose-dependent manner,thereby,play its role against hepatic fibrosis.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2002年第2期111-112,共2页
Chinese Journal of Vector Biology and Control
基金
湖北省卫生厅科学研究资金资助项目
