摘要
目的 :建立夏天无中原阿片碱和延胡索乙素的含量测定方法 ,为夏天无质量标准的研究提供科学依据。方法 :采用高效液相色谱法分离并测定了中药夏天无中原阿片碱和延胡索乙素的含量。色谱条件 :SupelcosilLC 18 DB柱 ,以甲醇 乙酸钠 /冰乙酸缓冲溶液 (75∶2 5 ,pH5 .0 )为流动相 ;检测波长 2 85nm。 4 86型紫外检测器检测。 结果 :此方法线性良好 ,平均加样回收率分别为 98.0 4 % ,99.6 3%。结论 :本方法精密度高 ,分离度良好 。
Objective:To establish the content determination method of the main alkaloids protopine and tetrahydropalmatine in Corydalis decumbens (Thunb.) Pers. Methods: HPLC was used to determinate the main alkaloids protopine and tetrahydropalmatine in Corydalis decumbene (Thunb.) Pers. Supelcosil LC 18 DB Column was used, mobile phase consisted of Methol NaAc/HAc(75∶25 pH5.0) and detection wavelength at UV 285 nm. Results: Linearity of this method was good with the average recoveries, 98.04% and 99.63%. Conclusion: This method is accurate, reliable with good separability and reproducibility, it can be applied as standard for the control of Corydalis decumbens (Thunb.) Pers.
出处
《中成药》
CAS
CSCD
北大核心
2002年第3期208-211,共4页
Chinese Traditional Patent Medicine
