摘要
目的:对长松萝多糖(PUS)清除氧自由基、抗脂质过氧化作用进行研究。方法:采用黄嘌呤-黄嘌呤氧化酶体系测定PUS对超氧阴离子自由基的清除作用,采用Fenton反应体系测定PUS对羟自由基的清除作用及其对羟自由基诱发的小鼠肝匀浆脂质过氧化反应的影响。结果:长松萝多糖对超氧阴离子自由基及羟自由基均有清除作用,其百分之五十抑制浓度IC_(50)分别为0.45 mg/ml和1.57 mg/ml,但对羟自由基诱发的小鼠肝匀浆脂质过氧化反应的抑制作用较弱。结论:PUS对氧自由基有明显的清除作用,但在Fenton反应体系中抗脂质过氧化作用较弱。
Objective: To study the scavenging action of polysaccharide of Usnea longissima. Ach. (PUS) to oxygen free radical and its inhibiting action on lipidperoxidation. Methods: The scavenging action to superoxide anion free radical (O2) was tested by xanthine-xanthine oxidase system. The scavenging action on hydroxyl free racical (OH·) and anti-lipidperoxidation effects were tested by Fenton reaction. Results : The scaverging action was represented by the content scavenging 50% of all the free radical (IC50) . The IC50 for O2 and OH· were 0.45 mg/ml and 1.57 mg/ml respectively. The lipidperoxidation induced by OH· was weakly inhibited by PUS. Conclusion: PUS can apparently scavenge oxygen free radical. But it can only weakly inhibit the lipid peroxidation of hepatocyte homogenate of mice which resulted form hydroxyl free radical.
出处
《中药材》
CAS
CSCD
北大核心
2002年第3期188-189,共2页
Journal of Chinese Medicinal Materials
关键词
长松萝多糖
清除
氧自由基
抗脂质过氧化反应
实验研究
Polysaccharide of Usnea longissima Ach.
Superoxide anion free radical
Hydroxyl free radical
Lipid peroxidadon
