摘要
我们在构建昆虫杆状病毒转移载体的过程中,发现了一个小质粒pQS145,其长2.0 kb,拷贝数很高,有EcoR I、Pst Ⅰ单一位点和3个Hinf Ⅰ位点,有抗氨苄青霉素基因,能与pBR 322探针杂交。用双酶消化法构建了此质粒3个酶的物理图谱。pQS 145是由pBR322缺失重组而来,实验结果与推导结果完全一致。
A plasmid, pQS 145, was extracted and purified from E. coli cells in construction of insect baculovirus transfer vector. Basic properties of the plasmid are as follows: 2.0 kb long for the dsDNA, high copies in E. coli cells, APr gene in the geneme, unique sites for EcoRI and Pst I, three sites for Hinf I stc. The plasmid can hybridize with pBR322 DNA probe. The physical map of the three restriction endonucleases in the pQS145 DNA was constructed. Therefore, the plasmid, pQS 145, was derived from the pBR322 by deletion recombination. The experimental results are consistent with those deduced according to deletion in pBR322 DNA.
出处
《同济医科大学学报》
CAS
CSCD
北大核心
1991年第5期299-302,共4页
Acta Universitatis Medicinae Tongji
关键词
质粒
杂合体
重组
基因
plasmid
hetezozygote
recombination
genetic