摘要
以云南省冰核细菌的优势种类丁香假单胞菌Pseudomonassyringae和菠萝泛菌Pantoeaananas为重组基因工程菌的受体菌 ,采用Tn5转座子诱变技术 ,以大肠杆菌EscherichiacoliS17/pZJ2 5∶∶Tn5作为转座子诱变的供体菌 ,使Tn5插入并整合到冰核细菌基因组中 ,使冰核基因的线性连续性被破坏 ,失去冰核活性。大量筛选接合子 ,获得 15
Using Pseudomonas syringae and Pantoea ananas , the dominant ice nucleation active ( INA ) bacteria strains from Yunnan province, as receptors of genetic engineering recombination, and Escherichia coli S17/pZJ25∶∶Tn5 as donator. By transposon Tn5 induced mutants method, the Tn5 was inserted and integrated into the genome of wild type INA + bacteria. INA + mutant as INA - when the linear continuous of INA + gene destroyed. 157 strains of INA - bacteria were obtained from numerous transconjugants.
出处
《云南农业大学学报》
CAS
CSCD
2002年第1期1-3,20,共4页
Journal of Yunnan Agricultural University
基金
云南省应用基础科学基金资助项目 (98C0 0 4M
98C0 33M)
